Skip to Main content Skip to Navigation
Journal articles

High-Density Lipoprotein Therapy in Stroke: Evaluation of Endothelial SR-BI-Dependent Neuroprotective Effects

Abstract : High-density lipoproteins (HDLs) display endothelial protective effects. We tested the role of SR-BI, an HDL receptor expressed by endothelial cells, in the neuroprotective effects of HDLs using an experimental model of acute ischemic stroke. After transient intraluminal middle cerebral artery occlusion (tMCAO), control and endothelial SR-BI deficient mice were intravenously injected by HDLs or saline. Infarct volume and blood-brain barrier (BBB) breakdown were assessed 24 h post tMCAO. The potential of HDLs and the role of SR-BI to maintain the BBB integrity was assessed by using a human cellular model of BBB (hCMEC/D3 cell line) subjected to oxygen-glucose deprivation (OGD). HDL therapy limited the infarct volume and the BBB leakage in control mice relative to saline injection. Interestingly, these neuroprotective effects were thwarted by the deletion of SR-BI in endothelial cells and preserved in mice deficient for SR-BI in myeloid cells. In vitro studies revealed that HDLs can preserve the integrity of the BBB in OGD conditions, and that this effect was reduced by the SR-BI inhibitor, BLT-1. The protection of BBB integrity plays a pivotal role in HDL therapy of acute ischemic stroke. Our results show that this effect is partially mediated by the HDL receptor, SR-BI expressed by endothelial cells.
Document type :
Journal articles
Complete list of metadatas

https://hal.sorbonne-universite.fr/hal-03105419
Contributor : Gestionnaire Hal-Su <>
Submitted on : Monday, January 11, 2021 - 10:02:00 AM
Last modification on : Wednesday, January 13, 2021 - 3:25:09 AM

File

ijms-22-00106-v2.pdf
Publication funded by an institution

Identifiers

Citation

Alexy Tran-Dinh, Angélique Levoye, David Couret, Lauriane Galle-Treger, Martine Moreau, et al.. High-Density Lipoprotein Therapy in Stroke: Evaluation of Endothelial SR-BI-Dependent Neuroprotective Effects. International Journal of Molecular Sciences, MDPI, 2021, 22 (1), pp.106. ⟨10.3390/ijms22010106⟩. ⟨hal-03105419⟩

Share

Metrics

Record views

95

Files downloads

84