Tandem MS Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra - Institut de Physique Nucléaire d'Orsay Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 2018

Tandem MS Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra

Résumé

Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrenolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these gamma-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified gamma-lactone, paving the way for future studies in biosynthesis.
Fichier principal
Vignette du fichier
AnalChem Sextonia 1.pdf (812.16 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02008867 , version 1 (18-08-2022)

Identifiants

Citer

Tingting Fu, David Touboul, Serge Della-Negra, Emeline Houël, Nadine Amusant, et al.. Tandem MS Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra. Analytical Chemistry, 2018, 90 (12), pp.7535-7543. ⟨10.1021/acs.analchem.8b01157⟩. ⟨hal-02008867⟩
188 Consultations
48 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More