IFN-γ-producing CD45RA+ CD8+ and IL-10-producing CD45RA- CD4+ T cells generated in response to LACK in naive subjects never exposed toLeishmania - Archive ouverte HAL Access content directly
Journal Articles European Journal of Immunology Year : 2002

IFN-γ-producing CD45RA+ CD8+ and IL-10-producing CD45RA- CD4+ T cells generated in response to LACK in naive subjects never exposed toLeishmania

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Eliane Bourreau
  • Function : Author
Marion Collet
  • Function : Author
Geneviève Milon
  • Function : Author
Dominique Ashimoff
  • Function : Author
Hitoshi Hasagewa
  • Function : Author
Carlos Parra-Lopez
  • Function : Author
Pascal Launois
  • Function : Author

Abstract

Leishmania guyanensis (L.g.)-specific CD8+ T cells can be isolated from PBMC of subjects who have never been previously exposed to Leishmania. Cells that produce IFN-gamma in response to live L.g. are generated from naive CD45RA+CD8+ T cells. The generation of L.g.-specific CD8+ T cells requires the presence of whole L.g. or UV-irradiated parasite but not the soluble antigens from L.g. promastigotes. The IFN-gamma-producing T cells recognize a specific antigen, the Leishmania homologue of receptors of activated C kinases (LACK) and this antigen but not live L.g. can produce a strong IL-10 response in CD45RA-CD4+ memory T cells from naive subjects. A single epitope (amino acid 156-173) is found to induce the IL-10 synthesis. While the IFN-gamma-producing cells are present among CD45RA+CD8+ T cells that are CD62L-CDR7- and CLA-, the LACK-reactive IL-10-producing cells are CD4+ T cells that are CD62L+CCR7- and CLA-.

Dates and versions

hal-03284784 , version 1 (12-07-2021)

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Eliane Bourreau, Marion Collet, Ghislaine Prévot, Geneviève Milon, Dominique Ashimoff, et al.. IFN-γ-producing CD45RA+ CD8+ and IL-10-producing CD45RA- CD4+ T cells generated in response to LACK in naive subjects never exposed toLeishmania. European Journal of Immunology, 2002, 32 (2), pp.510-520. ⟨10.1002/1521-4141(200202)32:2<510::aid-immu510>3.0.co;2-4⟩. ⟨hal-03284784⟩

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