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Journal articles
Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR
Abstract : A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.
https://hal.univ-guyane.fr/hal-03289316
Contributor : Mirlène Matillon <>
Submitted on : Friday, July 16, 2021 - 5:56:30 PM
Last modification on : Saturday, July 17, 2021 - 3:29:51 AM
Contributor : Mirlène Matillon <>
Submitted on : Friday, July 16, 2021 - 5:56:30 PM
Last modification on : Saturday, July 17, 2021 - 3:29:51 AM
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