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Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR

Abstract : A new method for specific reamplification of DDRT-PCR products is presented. After transient ligation of the primary DDRT-PCR fragments into a T-vector, the cDNAs of interest were reamplified by hemi-nested PCR and thermally asymmetric cycles. In contrast to the originally described protocol, this method of reamplification is specific, sensitive, reproducibly gives a high yield of DNA and allows direct sequencing of the reamplified product without purification or cloning.
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https://hal.univ-guyane.fr/hal-03289316
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Submitted on : Friday, July 16, 2021 - 5:56:30 PM
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S Bonnet, Ghislaine Prevot, C Bourgouin. Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR. Nucleic Acids Research, Oxford University Press, 1998, 26 (4), pp.1130-1131. ⟨10.1093/nar/26.4.1130⟩. ⟨hal-03289316⟩

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